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Identification and Differential Expression of Two Dehydrin cDNAs during Maturation of Jatropha curcas Seeds
，-0001，（）：
Plant dehydrin proteins (DHNs) are known to be important for environmental stress tolerance and are involved in various developmental processes. Two fulllength cDNAs JcDHN1 and JcDHN2 encoding two dehydrins from Jatropha cur cas seeds were identified and characterized. JcDHN1 is 764 bp long and contains an open reading frame of 528 bp. The deduced JcDHN1 protein has 175 a.a. residues that form a 19.3kDa polypeptide with a predicted isoelectric point (pI) of 6.41. JcDHN2 is 855 bp long and contains an open reading frame of 441 bp. The deduced JcDHN2 protein has 156 a.a. residues that form a 17.1kDa polypeptide with a predicted pI of 7.09. JcDHN1 is classified as type Y3SK2 and JcDHN2 is classified as type Y2SK2 according to the YSK shorthand for structural classification of dehydrins. Homology analysis indi cates that both JcDHN1 and JcDHN2 share identity with DHNs of other plants. Analysis of the conserved domain revealed that JcDHN2 has glycoside hydrolase GH20 superfamily activity. Quantitative real time PCR analysis for JcDHN1 and JcDHN2 expression during seed development showed increasing gene expression of both their transcript levels along with the natural dehydration process during seed development. A sharp increase in JcDHN2 transcript level occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. These results indicate that both JcDHNs have the potential to play a role in cell protection during dehydration occurring naturally during jatropha orthodox seed development.
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