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Cloning and Subcellular Localization Analysis of GhISR3, a Salt Tolerance Related Gene in Upland Cotton Seedlings

• 1、Hebei Agricultural University, Key Laboratory for Research and Utilization of Crop Germplasm Resources in North China, Ministry of Education, Baoding 071001

Abstract：Soil salinization is one of the important environmental stresses in agricultural production, which seriously affects the yield and quality of crops. Compared to the main food crops, cotton has higher salt tolerance and is also known as the pioneer crop for planting in saline alkali soil. Therefore, the development of cotton planting in saline alkali land can effectively alleviate the contradiction of "grain and cotton competing for land", and is also an important direction for the adjustment of China\'s cotton industry structure. It is also one of the specific measures to "continuously promote the transformation from mainly treating saline alkali land to adapting crops to more breeding of salt alkali tolerant plants to adapt to saline alkali land". However, there are significant differences in the sensitivity of cotton to salt stress at different stages of development, especially during the seedling stage, which is more sensitive to salt stress. Therefore, exploring salt tolerance related genes in cotton seedlings and analyzing their salt tolerance mechanisms has more theoretical and practical value for improving cotton salt tolerance and developing cotton planting in saline alkali soil. However, the progress of related research is slow. This study cloned a differential protein GhISR3 based on proteome sequencing of six cotton varieties during seedling stage under salt stress in the early stage. The open reading frame of this gene contains 228 bp and encodes 75 amino acids. Gene structure analysis shows that the gene has 3 exons and 2 introns. The protein product prediction analysis showed that the protein encoded by the gene had no signal peptide and transmembrane domain, and its isoelectric point was 4.763. Conservative domain analysis shows that GhISR3 belongs to the metallothionein family of proteins. Subcellular localization shows that GhISR3 is located in the nucleus.)

Keywords： Upland cotton Salt resistance Bioinformatics analysis Gene cloning.